Fig. 3
Impaired neuronal electric activities of Aβ1-42 and/or YKL-40 treated primary neurons. A Representative raw spike traces from four active electrode versus time after exposure to 10 μM Aβ, 400 ng/mL YKL-40, and 10 μM Aβ + 400 ng/mL YKL-40 in DIV18 cultures. B–C Quantified data showing time-dependent mean amplitude and spike rate for MEA neuronal cultures. Data are mean ± SEM of 100–120 neurons from three independent experiments. One-way ANOVA with Tukey’s post hoc comparisons. *p < 0.05, **p < 0.01, ***p < 0.001. D Confocal images (Mag. 10X) of primary neurons immuno-stained with anti-MAP2 (cyan signal), anti-PSD95 (green signal), and anti-SYP (red signal) antibodies. Scale bars, 100 μm. E–F Mean intensity and surface area were quantified. Mean surface area (n = 12), Density (n = 8) with at least 100 neurons included. Data are mean ± SEM. One-way ANOVA with Tukey’s post hoc comparisons. *p < 0.05, **p < 0.01