Fig. 4
Knockout of YKL-40 in astrocytes reduces Aβ deposition in 5xFAD mice brain. All mice were IP injected with 20 mg/mL tamoxifen in corn oil (100 µl per mice/per day) for 7 consecutive days before experiments. Experiments were carried out 7 days after last tamoxifen injection. A Confocal images (Mag. 10X) of dentate gyrus region of 5xFAD;Aldh;Chi3l1+/+; 5xFAD;Aldh;Chi3l1+/fl; 5xFAD;Aldh;Chi3l1fl/fl mouse brain immuno-stained with DAPI (blue signal), anti-YKL-40 (green signal), and anti-GFAP (red signal) antibodies. Scale bars, 100 μm. B Parallel brain sections were co-stained with DAPI (blue signal), anti-Aβ (green signal), and anti-GFAP (red signal) antibodies. C High magnification image of Dentate Gyrus regions. Quantification of plaque number from Aβ signals and mean surface area. Data are mean ± SEM. One-way ANOVA with Tukey’s post hoc comparisons. *p < 0.05, **p < 0.01, ***p < 0.001 compared to 5xFAD;Aldh;Chi3l1+/+