Fig. 5

Depletion of YKL-40 in astrocytes prevents neuronal loss in the brain of 5xFAD mice. All mice were IP injected with 20 mg/mL tamoxifen in corn oil (100 µl per mice/per day) for 7 consecutive days before experiments. Experiments were conducted 7 days after last tamoxifen injection. A Confocal images (Mag. 10X) of dentate gyrus region 7-m mice brain immuno-stained with DAPI (blue signal), anti-NeuN (green signal), and anti-GFAP (red signal) antibodies. Scale bars, 100 μm. B Mean NeuN + density were quantified. Data are mean ± SEM of 7–9 brains (2–3 slices for each brain), then analysed by One-way ANOVA with Tukey’s post hoc comparisons. ***p < 0.001, compared to 5xFAD mice. DG: dentate gyrus. C Western blotting analysis of neuronal proteins in lysates isolated from cortex (Cor) and hippocampus (Hip). GAPDH was used as an internal control. D Western results were quantified for cortical (Cor) and hippocampal (Hip) samples. Data are mean ± SEM. n = 7. One-way ANOVA with Tukey’s post hoc comparisons. *p < 0.05, **p < 0.01, ***p < 0.001 compared to WT mice