Fig. 2
MOG-Fc depletes anti-MOG antibodies but spares MOG-reactive B cells. a Kinetics of the depletion of anti-MOG antibodies in the serum of RR mice injected with MOG-Fc (n = 4) or SD-Fc (n = 3); RR mice were injected with 200 µg of MOG-Fc or SD-Fc and the sera were collected 3 days before injection, 4 h after injection, and on days 1, 3, 5 and 8 after injection. anti-MOG IgG ELISA was performed and OD at 450 nm (top panel), and percent change compared to day -3 (bottom panel) are shown. Each circle represents one mouse. **P = 0.0021 at 4 h, **P = 0.0012 at day 1 (Sidak’s multiple comparison test). b Depletion of anti-MOG antibodies in the serum of RR mice injected with MOG-Fc (n = 5), SD-Fc (n = 5). RR mice were injected with 200 µg of MOG-Fc or SD-Fc and the sera were collected 7 days before injection and on days 8 and 14 after injection. anti-MOG IgG ELISA was performed and OD 405 nm (top panel), and percent change compared to day -7 (bottom panel) are shown. One representative experiment out of 3 experiments performed is shown. Each circle represents one mouse. **P = 0.0026 (Sidak’s multiple comparison test). c Splenocytes from CD45.1+ IgHMOG SJL/J mice were transferred i.v. to CD45.2+ SJL/J mice which were subsequently injected with 200 µg of MOG-Fc (n = 6) or SD-Fc (n = 6). Experimental setup (top panel) and the frequencies of B cells (bottom panel) among the transferred CD45.1+ cells analyzed by flow cytometry 3 days after MOG-Fc and SD-Fc injection were shown. Each circle represents one mouse. Data from 2 experiments are pooled. Data are represented as mean ± s.e.m. d–g IgHMOG C57BL/6 mice received 200 µg of MOG-Fc (n = 4) or SD-Fc (n = 5) or rMOG (n = 4). After 3 days, lymph node cells were analyzed by flow cytometry. Each circle represents one mouse. The Mean Fluorescence Intensity (MFI) of CD21 (d), frequencies of CD5+ cells (e), frequencies of PDL1+ cells (f), and frequencies of PDL2+ cells (g) in IgHMOG B cells are shown. All data are represented as mean ± s.e.m. All experiments were performed at least twice