Fig. 3
Effect of MOG-Fc on antigen presentation and T cell functionality. a Proliferation of MOG-specific CD4+ T cells that were cocultured with IgHMOG B cells preloaded with MOG-Fc or SD-Fc (n = 4 per group). Representative flow cytometry plots (left panel) and the percentage of proliferating T cells (right panel) are shown. *P = 0.0286 (Mann–Whitney’s U test). b The proliferation of MOG-specific CD4+ T cells in vivo in the presence of antigen preloaded IgHMOG B cells. WT C57BL/6 mice received (i.v.) IgHMOG B cells preloaded with MOG-Fc (n = 8) or SD-Fc (n = 8), and one day later they received (i.v.) CD4+ T cells from OSE mice. Representative flow cytometry plots (left panel) and the percentage of proliferating T cells (right panel) are shown. Each circle represents one mouse. Data from 2 experiments are pooled. ***P = 0.0002 (Mann–Whitney’s U test). c The proliferation of MOG-specific CD4+ T cells in vivo in the presence of soluble MOG antigen and IgHMOG B cells. WT C57BL/6 mice received (i.v.) OSE splenocytes cells and 2 days later they were injected (i.p.) with 200 µg of MOG-Fc (n = 5) or SD-Fc (n = 5). Representative flow cytometry plots (left panel) and the percentage of proliferating T cells (right panel) are shown. Each circle represents one mouse. Data from 2 experiments are pooled. ***P = 0.0159 (Mann–Whitney’s U test). d–f WT C57BL/6 mice received (i.v.) OSE splenocytes along with 200 µg of MOG-Fc (n = 9) or SD-Fc (n = 8). After 3 days, lymph node cells were analyzed by flow cytometry. Each circle represents an individual mouse. Data from 2 experiments are pooled. d The frequencies of LAG3+ cells as a percentage of MOG-specific CD4+ T cells. *P = 0.0351 (unpaired T-test). e The frequencies of PD1+ cells as a percentage of MOG-specific CD4+ T cells; f the frequencies of Foxp3+ cells as a percentage of total CD4+ T cells. All data are represented as mean ± s.e.m. All experiments were performed at least twice