Fig. 5
From: Low-dose PLX5622 treatment prevents neuroinflammatory and neurocognitive sequelae after sepsis
Partial microglia depletion by low-dose PLX5622 prevents sepsis-induced synaptic elimination. A Representative 3D reconstructions and Airyscan processed confocal images of Homer1 postsynaptic terminals colocalized with Iba1-positive microglia. Scale bar 10 µm, 1 µm and 2 µm. B Quantitative analysis of colocalized Homer1 puncta and Iba1-positive microglia indicating synaptic engulfment or attachment of postsynaptic terminals by microglia in early systemic inflammation on day 3 (n = 4–6 animals/group, n = 3–4 microglia/animal, Two-way ANOVA with post hoc Tukey test). C Quantitative analysis of colocalized Homer1 puncta and Iba1-positive microglia indicating synaptic engulfment or attachment of postsynaptic terminals by microglia in chronic systemic inflammation on day 38 (n = 5 animals/group, n = 2 microglia/animal, Two-way ANOVA with post hoc Tukey test). Data are presented as mean ± SEM. Individual values are presented as small dots and each circle represents an average of one mouse; individual values and averages are color coded