Fig. 1

Characterization of the human primary olfactory mucosal cells at air–liquid interface. a Representation of the OM-ALI setup derived from human primary OM cells. b TEER measurement at days 7, 14, and 21 after initiation of OM-ALI cultures for cells derived from cognitively healthy control subjects. Graph shows mean with SD of n = 3 study subjects. Ordinary one-way ANOVA with Tukey’s multiple comparisons tests. **Indicates a p-value ≤ 0.005, *** indicates p-value ≤ 0.0005. c, d Representative immunostainings of OM-ALI cultures for the presence of zonula occludens-1 (ZO1), a tight junction marker; acetylated tubulin, a ciliary marker protein; and actin for the cytoskeleton of the cells. All imaged on 10 × objective; scale bar 100 µm