Fig. 2

Human OM-ALI express non-neural epithelial cells and receptor proteins required for SARS-CoV-2 infection. a Representative immunostainings of OM-ALI cultures for the presence mucin 5AC (MUC5AC) for goblet cells; Cytokeratin 18 (CK-18) for sustentacular cells; and actin for the cytoskeleton of the cells. All imaged on 10 × objective; scale bar 100 µm. b Quantification of the immunostainings representing the percentages of positive cells for MUC5AC for goblet cells; Cytokeratin 18 (CK-18) for sustentacular cells; and Ac-tubulin (acetylated tubulin) for ciliated cells. Graph shows mean with SD of n = 3 for cognitively healthy controls (total of 5 images were analyzed, each dot represents percentage of positive cell in single image). c Normalized filter counts of genes involved in SARS-CoV-2 infection, obtained from bulk mRNA sequencing of mock-treated OM-ALI cultures derived from cognitively healthy controls. Graph shows mean with SD of n = 3. d Representative immunostaining images of ACE-2 and NRP-1 expression in the OM-ALIs. Imaged ACE-2 on 63 × (scale bar 20 µm), and NRP-1 on 10 × objective (scale bar 100 µm)