Table 1 Key resources for immunostainings
Reagent or resource | Source | Identifier (catalogue number; lot number) |
|---|---|---|
Primary antibodies | ||
Monoclonal anti-tubulin, acetylated antibody produced in mouse; Dilution: 1:2000 | Sigma-Aldrich | T6793-100UL; 108923 |
Mouse monoclonal MUC5AC antibody (45M1); Dilution 1:200 | Thermo Fisher Scientific | MA5-12178; WD3205962 |
Mouse monoclonal human Cytokeratin 18 antibody (810811); Dilution 1:50 | R&D Systems | MAB7619; GR3268718-6 |
Goat anti-ACE-2 polyclonal antibody; Dilution 5 µg/mL | R&D Systems | AF933-SP; HOK0320051 |
Recombinant Rabbit monoclonal Anti-Neuropilin 1 antibody [EPR3113] Dilution 1: 250 | Abcam | ab81321; 212288-45 |
ZO-1 Polyclonal Antibody; Dilution: 5 µg/mL | Invitrogen | 40-2200; WA317222 |
SARS-CoV/SARS-CoV-2 Nucleocapsid Dilution: 1:2000 | Kind gift by Jussi Hepojoki | Cantuti-Castelvetri et al., Science, 2020 [18] |
Secondary antibodies | ||
Donkey anti-Goat Alexa Fluor-488 Dilution: 1:1000 | Invitrogen | A-11055; 2,513,496 |
Donkey anti mouse Alexa Fluor555 Dilution: 1:500 | Invitrogen | A-32773; VB302733 |
Goat anti rabbit Alexa Fluor-647 Dilution: 1:500 | Invitrogen | A-31573; 2497486 |
Alexa Fluor-488 conjugated phalloidin | Invitrogen | A-12379; 1948083 |
Hoechst DNA stain | Thermo Fisher Scientific | 62249; MF1423541 |