Fig. 1

ScRNA-seq analysis of marrow cells from the contralateral and ipsilateral skull after 15 min of stroke reperfusion. a Flow cytometry assay of mouse skull marrow myeloid cell proportions 15 min, 3 h, 6 h, 24 h, and 7 d after stroke reperfusion (n = 8 to 11 mice per group, one-way ANOVA with Tukey’s multiple comparison). The data are presented as the means ± SDs. ***P < 0.001, ****P < 0.0001, compared to the respective sham group. b The tSNE plots show the eight clusters of contralateral and ipsilateral skull marrow cells. c Proportion of cell types between the contralateral and ipsilateral groups. d Violin plots displaying the distribution and expression of marker genes of the 8 clusters. e Differentiation trajectory generated using Monocle 2 with stem cells. Pseudo-time values were calculated and plotted. f Sankey diagram showing the ratio of state 1, state 2, and state 3 stem cells between contralateral and ipsilateral sides. g Most enriched GO categories of the upregulated (ranked by –log10 (P value)) genes in B cells. h GO enrichment analysis of upregulated genes in monocytes. i GO enrichment analysis of upregulated genes in neutrophils