Fig. 2

Comparison of cell numbers and signals between contralateral and ipsilateral skulls. a Comparison of neutrophil proportions between ipsilateral and contralateral bone marrow in different tissues from male mice. The data are presented as the means ± SDs (paired t test, *P < 0.05, n = 6–7). b Comparison of monocyte proportions between ipsilateral and contralateral groups in the bone marrow of different tissues from male mice. The data are presented as the means ± SDs (paired t test, *P < 0.05, n = 6–7). c Comparison of B cell proportions between ipsilateral and contralateral groups in the bone marrow of different tissues from male mice. (paired t test, *P < 0.05, n = 6–8). d Comparison of Ki67 fluorescence intensity (normalized values) of LS-K cells in the skull marrow. The data are presented as the means ± SDs (paired t test, *P < 0.05, n = 6). e Single-cell RNA sequencing tSNE plots showing the distribution of CXCL12-positive expression in various cell types of the skull marrow. The distribution of CXCL12 genes is shown in red circles. f Violin diagram showing the expression level of CXCL12 between the ipsilateral and contralateral groups (P = 0.723). g Representative Western blot images of CXCL12 expression in the ipsilateral and contralateral skulls of the sham and stroke groups. h Comparison of relative CXCL12 expression levels (normalized to the sham group). The data are presented as the means ± SDs, *P < 0.05; ***P < 0.001. (Dunn’s multiple comparison test, n = 6). i Neutrophil to monocyte ratio of tibia marrow between the vehicle and SR59230A groups. The data are presented as the means ± SDs, *P < 0.05; (t test, n = 5–7). j Neutrophil-to-monocyte ratio of ipsilateral and contralateral skull marrow in the vehicle and SR59230A groups. The data are presented as the means ± SDs, *P < 0.05; (paired t test for the vehicle group, n = 5; Wilcoxon test for the SR5923A group, n = 7). k Membranes showing the change in cytokines between the ipsilateral and contralateral skull. l The intensities of the spots were quantified. Analysis showed significantly increased cytokines (BAFF, CCL3, CCL21, CD160, EGF, and others) and downregulation of VCAM-1