Fig. 3

OPN signaling in the region-specific activation of the skull. a Signaling pathways of overall information flow in the ipsilateral and contralateral skull. b Incoming communication patterns of cells. c Representative Western blot images of OPN expression in the contralateral and ipsilateral skulls of the vehicle and OPN inhibitor groups. d Comparison of relative OPN expression levels. The data are presented as the means ± SDs, *P < 0.05 (one-way ANOVA with Tukey’s multiple comparison test, n = 6). e Comparison of neutrophil proportions between different groups of skull marrow. The data are presented as the means ± SDs (paired t test, *P < 0.05, n = 6). f Comparison of monocyte proportions between different groups of skull marrow. The data are presented as the means ± SDs (paired t test, *P < 0.05, **P < 0.01; n = 6). g Comparison of leptomeningeal neutrophil proportions between different groups. The data are presented as the means ± SDs, *P < 0.05 (one-way ANOVA with Tukey’s multiple comparison test, n = 7). h Comparison of leptomeningeal Ly6C^Hi monocyte proportions between different groups. The data are presented as the means ± SDs. *P < 0.05, **P < 0.01. (one-way ANOVA with Tukey’s multiple comparison test, n = 7). i Quantitative analysis of mNSS scores. The data are presented as the means ± SDs. ***P < 0.005, ****P < 0.001 (two-way ANOVA with Bonferroni multiple comparison test, n = 10). j Quantitative analysis of foot fault percentages. The data are presented as the means ± SDs. *P < 0.05, **P < 0.01 (two-way ANOVA with Bonferroni multiple comparison test, n = 10)