Fig. 3

EMPA relieves inflammation and neuron death in IR retinas as assessed by single-cell analysis. A A total of 55,491 cells (Blank, 9282 cells; IR, 26,255 cells; and EMPA-treated IR mice, 19,954 cells) were retained for downstream analysis. t-SNE plot showing the distribution of different retinal cell types. B t-SNE plot showing the distribution of retinal cells from three groups. C Line charts showing the ratio alterations of different clusters of immune cells in all retinal cells. D Violin plot showing the inflammatory scores of three groups. For the box plot within each violin plot, the middle line of the box within the violin indicates the median value and the box ranges from the 25th to 75th percentile. E Ridge plots showing the inflammatory scores of different cell types. F Bar plots showing the GO terms enriched for the upregulated IR-DEGs (left) and downregulated EMPA-DEGs (right) of overall retinal cells. The color from light to dark indicates the statistical significance value from low to high. G GSEA of IR-DEGs mapping to GO pathway inflammatory response. H GSEA of EMPA-DEGs mapping to GO pathway neuron death