Fig. 5

EMPA attenuates LPS-induced inflammation and NLRP3 inflammasome activation in BV2 cells. A qRT-PCR analysis of mRNA expression levels of inflammatory factors (IL-1β, IL-6, TNF-α) in control and LPS-stimulated BV2 cells treated with or without EMPA (n = 4–6). B Western blotting analysis was carried out to detect NLRP3 inflammasome pathway-related proteins. β-Actin served as the internal protein control. C–G Relative densitometry quantitation of relative protein expression levels in the 3 groups of BV2 cells (n = 3–4)