Fig. 3

Therapeutic PLX3397 treatment (days 30–70 post-transplant) attenuates cGVHD-associated memory impairment. A Experimental timeline for bone-marrow transplantation, administration of either control or PLX3997-containing chow, and behavioural testing from day 70 post-transplant. B Schematic of visual cues and shock zone location used in the active place avoidance (APA) task. C Distance travelled in the APA paradigm on the day 0 (habituation day, shock zone off) between syngeneic mice and cGVHD mice, with and without PLX3397 treatment. D Entries into the shock zone for naïve mice showing spatial learning over a course of five testing days, which is unchanged by PLX3397 treatment compared with control chow. E Percentage improvement in APA performance on day 5 versus day 1 between syngeneic mice and cGVHD mice, with and without PLX3397 treatment. Note cGVHD mice on control chow had a significantly worse performance compared with naïve controls (dotted line, green asterisk), while the cGVHD treated with PLX3397 (150 ppm or 300 ppm) had a comparable APA performance compared to their syngeneic counterparts and naïve controls. F Schematic of open field task. G Distance travelled during the open field task. H Schematic of the elevated plus maze task. I Distance moved during the elevated plus maze task. J Time spent in the open arms during the elevated plus maze task. K Schematic of the phenomaster. Mice are provided a running wheel and food and water ad libitum. Various locomotion and metabolic measures are tracked by automatic sensors (represented by the black box). L Food intake measurement between syngeneic and cGVHD mice, with and without PLX3397 treatment. M Energy expenditure measurement between syngeneic and cGVHD mice, with and without PLX3397 treatment. N Respiratory exchange ratio measurement between syngeneic and cGVHD mice, with and without PLX3397 treatment. O Physical activity measurement between syngeneic and cGVHD mice, with and without PLX3397 treatment. P Running wheel activity of individual mice on days 5, 6 and 7 of the week in which they were given access to a running wheel. Statistics: one-way AONVA followed by Bonferroni post hoc comparisons (C, E, G, I, J, L–P), or two-way ANOVA with repeated measures and Bonferroni post hoc comparisons (D). APA: active place avoidance. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001