Fig. 4

Brain volume and diffusion tensor imaging (DTI) metrics in cGVHD mice and syngeneic transplant controls, with and without PLX3397 treatment. A Experimental timeline for irradiation, transplant and subsequent PLX3397 treatment. B, C Representative coronal image of ex vivo MRI T1/T2 flash image (B) and hippocampal volume (C). D Ex vivo sagittal image of fractional anisotropy (FA) displaying the hippocampal commissure (hpc), corpus callosum (cc) and anterior commissure anc). E–G Fractional anisotropy (FA) of the hippocampal commissure (E), corpus callosum (F), and anterior commissure (G). H–J Axial diffusivity (AD) of the hippocampal commissure (H), corpus callosum (I), and anterior commissure (J). K–M Radial diffusivity of the hippocampal commissure (K), corpus callosum (L), and anterior commissure (M). Dots represent individual mice. B, D Scale bars: 1 mm. Statistics: two-way ANOVA with Bonferroni post-hoc comparisons (C, E–M). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001