Fig. 2

Impact of SCI and O₂ therapy on the spinal cord transcriptome. A Data were generated by performing RNAseq on C4 spinal tissues, followed by hierarchical clustering of Weighted Gene Co-Expression Network Analysis (WGCNA) module eigengenes. In panel A, “Height” indicates the relative difference between modules, and the branches of the dendrogram group together eigengenes that are positively correlated. B The relative expression of each module, in each experimental group. Each row corresponds to a module eigengene, and each cell contains the corresponding correlation and p-value. Red and blue color denote positive and negative correlation with gene expression, respectively. C The functional classification of each module and number of associated genes. D, F Heat maps depict expression of the top 25 hub genes (rows) across animals (columns) for the six experimental groups (red corresponds to gene upregulation and blue to downregulation). These plots are provided for the blue module (“neuronal”, D) and turquoise (“inflammatory”, F) module. Bar plots above the heat maps show the overall expression level within each animal. E, G Boxplots are provided to illustrate variability in the expression levels of representative hub genes in the blue (top) and turquoise (bottom) modules. * indicates p < 0.05, student’s t-test compared to SCI