Fig. 3
From: Oxygen therapy attenuates neuroinflammation after spinal cord injury
Impact of O2 therapy on histological assessment of neuroinflammation near the spinal cord lesion. Representative photomicrographs depicting IBA1 and GFAP immunostaining are shown in C4 spinal cord transverse sections obtained from a spinal intact (panel A) and injured rat (SCI group, panel B). Ai and Bi show increased magnification views of the area highlighted by the box in A and B. Panels C and D provide quantitative evaluation of IBA1 + optical density on the ipsilateral and contralateral sides of the spinal cord, respectively. Immunostaining was evaluated ± 10 mm from C4 (lesion epicenter). Panels E and F present GFAP + optical density in the ipsilateral and contralateral spinal cord. A one-way analysis of variance was used to compare groups. The omnibus effect of group p-value is noted in the figure; *indicates p < 0.05 Tukey–Kramer post-hoc compared to Intact