Fig. 4
From: Oxygen therapy attenuates neuroinflammation after spinal cord injury
Impact of O2 therapy on vacuolization and neuronal numbers in the injured spinal cord. Representative photomicrographs of FluoroMyelin stained transverse C4 spinal cord sections are presented in panel A (spinal intact) and panel B (SCI group). Panels Ai and Bi show increased magnification views of the areas highlighted by the box in A and B. Panels B and Bi illustrate the increase in vacuolization in tissues from the injured spinal cord. Mean data were assessed ± 2 mm from the C4 lesion epicenter and are presented for the ipsilateral (panel C) and contralateral spinal cord (panel D). Panels E–F show example photomicrographs of the C4 spinal cord depicting NeuN staining (to recognize neurons) in the spinal intact (E) and injured spinal cord (F). Ei and Fi show increased magnification views of the areas highlighted by the box in E and F. Panel G shows group data for small neurons (23–115 µm2) and panel H shows large neurons (116–345 µm2) in the ventral horn on the ipsilateral side. In both cases, although values are lower, neuronal counts in the 3 ATA group are not statistically different than spinal intact. One-way analysis of variance was used to compare the groups; if a data-set failed ANOVA assumptions a Kruskal–Wallis (KW) test was used. The omnibus effect of group p-value is noted in the figure; *indicates p < 0.05 Tukey–Kramer post-hoc compared to Intact