Fig. 2

Effect of iNO on downstream signaling molecules. A Illustration depicting the NO signaling pathway. B–F Quantification of sGCα mRNA (B), sGCβ mRNA (C), cGMP levels (D), PKGα mRNA (E), and PKGβ mRNA (F) measured in the ipsilateral hemisphere in the indicated mice, expressed relative to the contralateral hemisphere. G Illustration depicting the strategy used to isolate and purify cerebral vessels (created using BioRender). H–J Representative western blot (H) and quantification (I and J) of the indicated proteins measured in vessels isolated from the indicated groups (c, contralateral; i, ipsilateral). K, L Quantification of eNOS and nNOS mRNA levels measured in the ipsilateral cerebral cortex in the indicated groups, expressed relative to the contralateral hemisphere. *p < 0.05 one-way ANOVA; n = 4–8 per group