Fig. 5

Foxg1 is a crucial regulatory molecule for CB2R-mediated autophagy promotion. A Principal Component Analysis (PCA) plot depicting the transcriptomic profiles of (specify the samples or conditions being compared). Each data point represents an individual sample, and the plot reveals the multidimensional relationships between samples based on their gene expression patterns. B Differential genes between LPS + ATP vs LPS + ATP + JWH133 groups (downregulated genes on the left, upregulated genes on the right). C The study involves retrieving the intersection between genes in a database and genes associated with sequencing changes. Subsequently, approximately 20 genes from this intersection will be selected for qPCR analysis. D Location of foxg1 among differentially expressed genes in volcano plot. E Western blot analysis was performed to assess the expression of foxg1. The corresponding representative blot is presented in F (for 4 independent experiments.). G Immunofluorescent staining of primary astrocytes (foxg1 is green, GFAP is red), and the fluorescence intensity statistics of foxg1 are shown in H (for 3 independent experiments). NS means not significant, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 compared with the corresponding group, as determined by the one-way ANOVA