Fig. 6

Foxg1 is a crucial regulatory molecule for CB2R-mediated autophagy promotion. A Protein detection of NLRP3 and MAP1LC3B in primary astrocytes. Statistical data for the proteins are presented in B, C, respectively (for 3 independent experiments). D Immunofluorescence staining of primary astrocytes showing GFAP (green fluorescence) marking astrocytes and NLRP3 protein (red fluorescence) detection. Fluorescence intensity data for NLRP3 are shown in E (for 3 independent experiments). F Expression levels of MAP1LC3B mRNA detected using Q-PCR (For 3 independent experiments). G Potential binding sites of foxg1 on the promoter region of MAP1LC3B. Three putative binding sites of foxg1 on the promoter region of MAP1LC3B were identified using cut&run assay (H–J, for 3 independent experiments). NS means not significant, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 compared with the corresponding group, as determined by the one-way ANOVA