Fig. 8

Knockdown of foxg1 hampers autophagic response in astrocytes and inhibits NLRP3 degradation. A Detection of protein levels for NLRP3, Caspase1, pro-Caspase1, IL-1β, and pro-IL-1β in the midbrain tissue by western blot, with quantification shown in panels B–D (n = 4). E Immunofluorescence staining for GFAP (green) and NLRP3 (red) in midbrain tissue of mice, with quantification shown in panel F–H (n = 4). G Mouse midbrain GFAP (red) and MAP1LC3B (green) immunofluorescence staining, the captured image was processed by imaris software, and the fluorescence statistics are shown in H, I (n = 4). J Autophagosomes (marked by red arrows) observed by transmission electron microscope in mouse midbrain tissue. NS means not significant, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 compared with the corresponding group, as determined by the one-way ANOVA