Skip to main content
Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Oxidative stress and inflammation cause auditory system damage via glial cell activation and dysregulated expression of gap junction proteins in an experimental model of styrene-induced oto/neurotoxicity

Fig. 1

Workflow of the experimental design. Male adult Wistar rats were randomly assigned at the beginning of the study to three experimental groups: “Ctrl”, “Styrene” and “Ctrl-gavage” groups. Baseline hearing thresholds were evaluated in all animals the day (D) before starting styrene treatment (D-1) by recording auditory brainstem responses (ABR). Animals of Styrene group underwent oral gavage treatment with styrene (400 mg/kg) for 3 weeks (from D0 to D21), once daily, 5 days a week. At the same time, Ctrl-gavage group underwent vehicle delivery (olive oil). At the end of the treatment (D21), after ABR recordings, animals were scarified and tissues, both cochlea and auditory cortex (ACx), were collected to perform experimental analyses. fEPSP: field excitatory postsynaptic potentials; WB: western blot; IF: immunofluorescence; Golgi: Golgi–Cox staining

Back to article page

Journal of Neuroinflammation

ISSN: 1742-2094