Fig. 6
From: Bruton’s tyrosine kinase-bearing B cells and microglia in neuromyelitis optica spectrum disorder
RNA sequencing reveals microglia changes in response to NMO–IgG injection and BTKi treatment. A–C Volcano plot of differential expression genes (DEGs) of microglia between NMO–IgG recipient and control–IgG recipients (A), between NMO–IgG recipient with BTKi treatment or not (B), between NMO–IgG recipient with BTKi treatment and control–IgG recipients (C). D GO pathway analysis for biological processes (red), molecular functions (green) and cellular components (blue) enrichment of DEGs of microglia from untreated NMO models and NMO models with BTKi treatment. E Heatmap showing specific genes involved in the biological processes ‘Cell adhesion’, angiogenesis and ‘ameboidal-type cell migration’ (scale = Z scores of FPKM values). F Venn diagram showing overlap between genes in the top 5 biological process. The NMO–IgG + BTKi represented NMO–IgG recipient mice with the treatment of 20 mg/kg zanubrutinib