Fig. 4

Effect of SUCNR1 inhibition on the Mφ phenotype. Mφs were treated with siRNAs and 1 mM succinate for 48 h and divided into four groups: siNC, siNC + succinate, siSUCNR1 and siSUCNR1 + succinate. A Relative RNA expression of iNOS, TNFα, IL6, TGF-β, CD206 and Arg1 in the four groups (n = 3). B–G Relative protein levels of SUCNR1, TGF-β, Arg1, IL6 and TNFα in the four groups (n = 3). H Immunofluorescence staining of Mφs with DAPI (blue), TGF-β (green) and Arg1 (red). Scale bar: 50 μm. I, J Relative fluorescence intensity of TGF-β and Arg1 (n = 3). K Flow cytometry analysis of Mφs in the four groups. L The ratio of CD206⁺/CD86⁺ cells in Mφs (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001