Fig. 6

Knockdown of SUCNR1 reduced the severity of succinate-induced CNV and RNV in animals. C57 mice were randomly divided into four groups: control, CNV + shNC, CNV + shNC + succinate, and CNV + shSUCNR1 + succinate. (A, top row) B Choroidal IB4 staining and areas of CNV in four groups on day 7 after Matrigel induction. Scale bar: 200 μm (n = 3). (A, second row) H&E-stained sections of eyeballs. Scale bar: 100 μm. (A, third and fourth row) FA images of four groups. C, D Waveform and amplitude of ERGs in each group (n = 3). E–J Protein levels of SUCNR1, TGF-β, Arg1, IL6 and TNFα in animals (n = 3). K The Arg1/IL6 protein level ratio (n = 3). L–N Retinal IB4 staining and size of neovascular area as well as avascular area in OIR models. Scale bar: 400 μm (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001