Fig. 4

Histopathologic analysis of the ovaries upon EAE. A, B Bar plots showing the distributions of weight and gonado-somatic index values for ovaries isolated from EAE and control mice at 20 dpi. C Representative H&E sections at low and high magnification of ovaries from EAE and control mice at 20 dpi. Magnification = 10 ×, scale bar = 200 μm (top) and magnification = 40 ×, scale bar = 50 μm (bottom). D, E Quantification of viable follicles and corpora lutea between EAE and control mice. F Quantification of viable follicles within each maturation stage between EAE and control mice. Differences between experimental groups (N = 5–6 per group from two EAE immunizations) were assessed by Mann–Whitney U-test. *P ≤ 0.05, **P ≤ 0.01; ***P ≤ 0.005