Fig. 4
CXCL9/CXCL10 induction is inhibited by LXA4 and LXB4 treatment and may interact with the CXCR3 receptor in the inner retina. A Heatmap of mean multiplex array results for 32 cytokines showing log2 fold inhibition of LPS-induced levels by LXA4 or LXB4. CXCL9 and CXCL10 were the top hits inhibited by each lipoxin (NC; not calculated due to levels being out of range). B Concentrations of CXCL9 and CXCL10 were significantly increased in retinal extracts at 24 h following LPS challenge compared to vehicle. However, eyes pre-treated with LXA4 or LXB4 showed significantly reduced induction of CXCL9 or CXCL10 levels compared to vehicle (*p < 0.05, **p < 0.01, ***p < 0.005). C The shared CXCL9/CXCL10 receptor, CXCR3 (green) is localized to the inner retina (arrows), and appeared induced at two days following LPS challenge. D CXCR3 staining partially co-localizes with the RGC marker Brn3a (red, arrows), and E CXCR3 (green) also partially co-localizes with GFAP (red, arrows). (Scale bars represent 100 µm, graph bars represent SE)