Fig. 2

Splenectomy inhibits acute subretinal inflammation independently of lymphocytes. A and B Quantification of CD11b⁺Ly6C^high classical monocytes in the blood (A) and of CD45^highCD11b⁺ monocyte-derived cells (MdCs) in the retina/choroid (B) of un-lasered controls and day4-lasered mice that have undergone splenectomies or sham-operations 30 days prior (A one-way ANOVA, un-lasered Ctl sham (n = 12) vs d4-lasered Sham (n = 10): $p = 0.0094; d4-lasered Sham (n = 10) vs d4-lasered Splenectomy (n = 8), *p = 0.046; B one-way ANOVA, un-lasered Ctl sham (n = 20) vs d4-lasered Sham (n = 10): ^$p =  < 0.0001; d4-lasered Sham (n = 10) vs d4-lasered Splenectomy (n = 10), *p = 0.037). C Representative images of 7d-laser injured IBA1 (green) and CD102 (red) stained RPE flatmounts of mice that have undergone splenectomies or sham-operations 30 days prior. Quantification of IBA1⁺ MPs surrounding laser impacts and the CD102⁺ CNV area (Mann–Whitney test, Sham (n = 10) vs splenectomy (n = 18), MPs *p = 0.0056; CD102⁺ area *p < 0.0001). Scale bar = 20µm. D Representative images of IBA1 (green, MP marker) and CD102 (red) stained RPE flatmounts of – 30 d sham-operated wild-type and sham-operated and splenectomized Rag2^−/− mice 7 days after laser impact. Quantification of IBA1⁺ MPs surrounding laser impacts and CD102⁺ CNV area (n = 10/group; one-way ANOVA, Sham vs Rag2^−/− splenectomy, MPs *p = 0.033 and CD102⁺ area *p = 0.019). Scale bar = 20 µm. Ctl: control; MP: mononuclear phagocyte; RPE: retinal pigment epithelium