Fig. 6

C-Mito^HF promoted cGAS-dependent pro-inflammatory cytokines production in ECs of the SFO in HF mice. A–C Double-immunofluorescent staining showed that compared with C-Mito^Ctrl, C-Mito^HF exacerbated endothelial expression of pro-inflammatory cytokines TNF-α in the SFO of HF mice, which could be mitigated by endothelial cGAS KD in the SFO. Scale bar = 100 μm. D–F Double-immunofluorescent staining showed that compared with C-Mito^Ctrl, C-Mito^HF exacerbated endothelial expression of pro-inflammatory cytokines IL-1β in the SFO of HF mice, which could be mitigated by endothelial cGAS KD in the SFO. Scale bar = 100 μm. n = 8, P < 0.05, ANOVA LSD test