Fig. 6

GFP is highly colocalized with proprioceptor markers in the DRG of Atf3:BAC Tg mouse after RCS. a–f Double immunostaining of GFP with various sensory neuron markers. IB4 is a marker of nociceptive neurons (a), CGRP is a marker of nociceptive neurons (b), TrkA is a marker of nociceptive neurons (c), TrkB is a marker of tactile/pressure sensation (d), TrkC is a marker of proprioceptor neurons (e), and VGluT1 is a marker of proprioceptor neurons (f). Note that co-localization can be strongly observed in e and f (arrowheads). g Co-expression rates of each marker with GFP-positive neurons in the DRG. n = 5 mice, *p < 0.001 vs. TrkC, **p < 0.0001 vs. TrkC, †p < 0.05 vs. VGluT1, ††p < 0.0001 vs. VGluT1. One-way ANOVA with Dunnett’s multiple comparisons test. Error bars denote the SEM. Scale bars, 40 μm