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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Synergistic effect of sildenafil combined with controlled hypothermia to alleviate microglial activation after neonatal hypoxia–ischemia in rats

Fig. 2

Histological brain lesions in P13 rats subjected to HI at P11 and kept either on normothermia (HI, n = 32) compared to sham (n = 9), or treated by hypothermia alone (HT, n = 17), Sildenafil ip alone (Sild, n = 23), and the combined treatment (Sild + HT, n = 23). Ipsilateral tissue loss (A, B) and hippocampal surface (C) in right hemisphere were normalized to contralateral hemisphere in Nissl staining. Mean % (SD) of tissue loss (A, C) or mean (SD) hippocampal surface (B) were measured by assessing the entire brain from Bregma + 2.52 mm to − 7.20 mm. In A and C, sham vs untreated (NT) HI animals were first compared using a non-parametric Mann Whitney t-test (****p < 0.0001). Then, each treatment was compared to untreated animals using a Kruskal–Wallis test (ns: non-significant). In B, distribution of lesion size groups was analyzed in each treatment group compared to HI using Chi-square for trend test (***p < 0.001; **p < 0.01)

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Journal of Neuroinflammation

ISSN: 1742-2094