Fig. 5

Gene expression polarization in microglial or astroglial cells sorted from P13 rats subjected to HI at P11 and kept either on normothermia (HI, n = 41) compared to sham (n = 12), or treated by hypothermia alone (HT, n = 19), Sildenafil ip alone (Sild, n = 20), and the combined treatment (Sild + HT, n = 18). Data were summarized as changes observed in each category of markers, either in ipsi or contralateral hemisphere. Mann–Whitney test was performed for Sham vs HI untreated groups comparison and a Kruskal–Wallis test followed by a Dunn’s multiple comparison test to compare HI untreated group and each of the HI treated groups (Sild, HT and SildHT). Number of arrows corresponds to direction and statistical difference magnitude (one arrow: p < 0.05; 2 arrows: p < 0.01; 3 arrows: p < 0.001; 4 arrows: p < 0.0001; ns: non-significant)