Fig. 7
Over-representation analysis (ORA) against the Reactome of the proteome of microglial cells sorted from the ipsilateral hemisphere of P13 rats subjected to HI at P11 without and with neuroprotective treatment Sild + HT. This ORA analysis was performed to identify the enriched Reactome pathways inversely regulated by HI and in HI-injured animals treated by Sild + HT (A). Reactome pathways defined by logFC > 1,3, p-value < 0.05 and FDR < 0.25 were considered significantly up or downregulated. Three reactome pathways were identified (B–D) among them common proteins were also inversely deregulated (highlighted in orange). A sensitivity analysis using ORA geneontology/Biological Process noredundant was also performed. Overall proteins inversely deregulated in each analysis were summarized in E and a network of these proteins was obtained using String (http://string-db.org/) with a confidence threshold fixed at 0.4 (medium) (F). This network shows significantly more relations than expected by chance (5 nodes, 7 edges while none was expected, p-value = 2.6–11)