Fig. 1

SRGN is remarkably elevated after focal ischemia, especially in microglia. A Violin plot showing the total expression of Srgn gene from the ischemic brain at different time points (Sham, 3 h, 12 h, 3d) after MCAO according to the scRNA-seq data from our previous work. B The qPCR analysis of Srgn mRNA levels at different time points (Sham, 3 h, 6 h, 12 h, 1d, 3d, 7d) after MCAO. C Representative immunoblot image showing the protein level of SRGN at different time points (Sham, 3 h, 6 h, 12 h, 1d, 3d, 7d) after MCAO. β-Actin served as the loading control. D The statistical analysis of brain homogenate SRGN level after MCAO 1 day. n = 3 ~ 4 mice per group. E Representative immunofluorescence images of SRGN expression in cortex neurons (NeuN⁺), astrocytes (GFAP⁺) and microglia (Iba1⁺) of mice after MCAO 1 day. Scale bar, 30 μm. F Quantification of co-localization of SRGN with NeuN, GFAP or Iba1 in cortex of mice 1 day after MCAO. n = 3 mice per group. G The statistical analysis of serum SRGN level at MCAO 1 day with or without the pretreatment of microglia scavenger PLX5622. H Violin plot showing the microglial expression of Srgn gene from the ischemic brain tissue at different time points (Sham, 3 h, 12 h, 3d) after MCAO according to the scRNA-seq data from our previous work. I Heat map showing part of the DEGs of sorted microglia from the ischemic brain tissue after MCAO 3 days according to the bulk-RNA seq data from our previous work. J The FPKM of Srgn according to the bulk-RNA seq data from our previous work. K The qPCR analysis of Srgn mRNA levels in primary microglia at different time points (0 h, 1 h, 3 h, 6h, 12 h) after LPS (100 ng/ml) stimulation. Data represented as mean ± SEM, * p < 0.05, ** p < 0.01, ****p < 0.0001, #p < 0.05