Fig. 6

SRGN exacerbates microglial inflammation in a CD44-dependent manner. A, B Representative images of TTC-stained brain sections (A) with the quantification of infarct volume (B) at 1 day after MCAO. Cd44-KO mice and their WT littermates were injected with rSRGN (2.5 mg/ mL) or control solvent. n = 5 ~ 6 mice per group. C, D The mNSS (C) and grip strength (D) performance of Cd44-KO mice and their WT littermates at 1 day after MCAO. n = 5 ~ 6 mice per group. E, F The qPCR analysis of Il1b and Tnf mRNA levels from brain tissue of Cd44-KO mice and their WT littermates at 1 day after MCAO, injected with rSRGN (2.5 mg/ mL) or control solvent. G Representative immunofluorescence images showing the morphology of microglia (Iba1⁺, green) in ischemic brains of WT mice injected with glycerol/ rSRGN and Cd44-KO mice injected with rSRGN 1 day after MCAO. H, I Quantification of the average branches (H) and process length (I) of microglia based on the images in G. Data represented as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001, ns, no significant