Fig. 3

RNA-seq analysis of bulk transcriptomes reveal transcriptional regulation associated with inflammation, synaptic function, and disease biology. RNA-seq analysis of bulk tissue transcriptome was performed at DIV6. A Volcano plot of differential gene expression. B Gene set enrichment analysis of significantly dysregulated genes was performed to identify salient biology. − Log₁₀[adj p-val] for enriched Kegg pathways is shown. Significant functional pathways are grouped below the histogram to highlight commonalities in dysregulated biology. C Transcription factor enrichment was assessed in genes that were significantly differentially expressed. A summary of the transcription factors, number of distinct motifs, and the number of genes that contain these motifs in their promoter region is shown to the right of the summary Venn diagram