Fig. 2
From: Th1 cells contribute to retinal ganglion cell loss in glaucoma in a VCAM-1-dependent manner
T helper 1 (Th1) response is predominant in patients with glaucoma. A Flow cytometric gating strategy to identify CD4+ T cell subsets in PBMC. PBMC were collected from GL and HC. CXCR3, CCR6 and CCR4 were used to determine the Th1 (CXCR3+), Th2 (CXCR3− CCR6− CCR4+), and Th17 (CXCR3− CCR6− CCR4+) subset of live CD4+ T cells. Representative flow cytometric plots were shown. B Comparison of Freq. Th1 between GL (n = 113) and HC (HC, n = 121). C Shown is comparison of Freq. Th1 among different groups stratified according to visual damage stage based on H-P-A classification system (Mild, n = 33; Moderate, n = 14; Severe, n = 66). D, E Comparison of D Freq. Th17 and E Freq. Th2 between GL and HC. **P < 0.01, ***P < 0.001, ns, no significance. Statistical comparisons were performed using B, D, E Mann–Whitney test; C, Kruskal–Wallis test followed by Dunn's multiple comparisons test