Fig. 3

Acetate inhibited neuroinflammation in rostral ventrolateral medulla (RVLM) in SHRs. A, immunofluorescence photomicrographs showing triple labelling of c-Fos (red), tyrosine hydroxylase (TH, green) and neuronal nuclei (NeuN, white) in RVLM (bregma: −12.3 mm) of each group. B-E, the number of NeuN⁺, TH⁺, TH⁺c-Fos⁺ and NeuN⁺c-Fos⁺ neurons in each group. F-H, relative expression of inflammatory factors such as interleukin 1 beta (Il1b), interleukin 6 (Il6) and tumor necrosis factor (Tnf) mRNA in the RVLM of rats in various groups by qPCR. n = 5 for each group. * P < 0.05, ** P < 0.01, *** P < 0.001