Fig. 2
Temporal gene and protein expression changes after retinal detachment and sequencing validation. (A) Heatmap showing significantly upregulated (FC ≥ 1.5, padj≤0.05, red) and downregulated (FC ≤ 0.67, padj≤0.05, blue) DEGs 1 and 7 dprd. (B-F) Relative expression values of DEG grouped by temporal expression. (B) Log2FC of all DEGs significantly upregulated at both 1 and 7 dprd (Up/Up). (C) Log2FC of DEGs significantly upregulated only at 1 dprd (Up/NSC). Gray indicates DEG that were not significantly changed (NSC). (D) Log2FC of DEGs significantly upregulated only at 7 dprd (NSC/Up). (E) Log2FC of DEGs significantly downregulated only at 1 dprd (Down/NSC). (F) Log2FC of DEGs significantly downregulated at both 1 and 7 dprd (Down/Down). (G-K) QRT-PCR of temporal DEG expression changes after retinal detachment for RNA-Seq validation. Relative mRNA expression of (G) Grem1, (H) S100a8, (I) Serpine3, (J) Rpe65, and (K) Tsc22d3, obtained from isolated naïve RPE (n = 6), as well RD RPE at 1 dprd (n = 6) and 7 dprd (n = 6). Bar graphs represent mean ± SEM. Statistical analysis was performed using one-way ANOVA with repeated measures followed by Tukey’s post hoc test. *p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001