Fig. 4
Pharmacological inhibition of NOX2 attenuates Pathologically high intraocular pressure-induced glaucomatous RGC loss and ON degeneration but not IOP modulation. (A) Representative images of WT and Nox2−/− retinas immunostained with Brn3a after Pressure 60 mmHg 24 h. Scale bar, 50 μm. (B) Analysis of the number of Brn3a-positive RGCs in the different groups. Data are shown as mean ± SEM (n = 6 in each group, one-way ANOVA with Tukey’s multiple comparisons test, *p < 0.05, **p < 0.01,***p < 0.001, ****p < 0.0001). (C) The timeline of C57BL/6J mice after H-IOP with gp91ds-tat treatment and IOP measurement. (D) IOP measurements revealed sustained significant IOP elevation in H-IOP mice with gp91ds-tat-injected compared to Veh-injected control mice. Data are shown as mean ± SEM (n = 6 in each group, two-way ANOVA with Tukey’s multiple comparisons test, ****p < 0.0001). (E) Representative images of flat mount retina immunostained with Brn3a after H-IOP treated by 500µM gp91ds-tat. Scale bar, 50 μm. Representative images of PPD-stained ON after H-IOP treated by 500µM gp91ds-tat, at low (upper row, scale bar, 20 μm) and high (lower row, scale bar, 5 μm) magnification. (F) Analysis of the number of Brn3a-positive RGCs of retinas treated by different concentrations of gp91ds-tat after H-IOP in the different groups. (G) Analysis of the number of axons of ON treated by different concentrations of gp91ds-tat after H-IOP in the different groups. (H) Analysis of the percent of degenerating axons of ON treated by different concentrations of gp91ds-tat after H-IOP in the different groups. Data are shown as mean ± SEM (n = 6 in each group, one-way ANOVA with Tukey’s multiple comparisons test, *p < 0.05, **p < 0.01,***p < 0.001, ****p < 0.0001). (I) Representative images of flat mount retina immunostained with Brn3a after Pressure 60 mmHg treated by 300µM gp91ds-tat. Scale bar, 50 μm. (J) Analysis of the number of Brn3a-positive RGCs of retinas treated by different concentrations of gp91ds-tat after Pressure 60 mmHg in the different groups. Data are shown as mean ± SEM (n = 6 in each group, one-way ANOVA with Tukey’s multiple comparisons test, *p < 0.05, **p < 0.01,***p < 0.001, ****p < 0.0001)