Fig. 3

miR-142-5p was increased in the hippocampus of rats in the CCI-associated memory impairment model. (A) Differentially expressed miRNAs were selected from sequencing data of hippocampal EV miRNAs. (B) Venn analysis of the seven candidate miRNAs satisfying the three conditions. (C) RT‒qPCR showed that the relative expression of mature miR-142-5p, mature miR-25-5p, mature miR-505-3p, and mature miR-873-5p were obviously upregulated in the hippocampus of CCI rats compared with that in sham rats. n = 4–6 rats. unpaired Student’s t test was used. (D) RT‒qPCR results showing that the relative expression of pri-miR-25-5p, pri-miR-505-3p and pri-miR-873-5p were downregulated in the hippocampus of CCI rats, but that of pri-miR-142-5p was upregulated compared with that in sham rats. n = 4–5 rats. unpaired Student’s t test was used. (E) RT‒qPCR results showing that the relative expression of mature miR-142-5p and miR-25-5p were increased in the sciatic nerves of CCI rats, but that the expression of mature miR-505-3p was decreased; moreover, there was no difference in the expression of mature miR-505-3p compared with that in sham rats. n = 4–6 rats. unpaired Student’s t test was used. The data are shown as the means ± SDs. ns, no significant difference; *P < 0.05; **P < 0.01