Fig. 4
IgM anti-GM2 binding on SCs results in complement activation. IgM binding (FCIgM, A) and C3 fixation (FCC3, B) to SCs opsonized with IgM anti-GM2 positive (red bars) or negative (black bars) MMN patient serum and incubated with fresh serum as complement source. Opsonization with anti-GM2 MMN patient serum results in increased IgM binding and increased complement activation. Data are mean + SD of different assays (C) Pooled results of FCC3 data depicted in (B), Mann-Whitney test. (D) Highly significant strong correlation between IgM anti-GM2 binding to SCs and subsequent complement activation. Red dots IgM anti-GM2 + sera, black dots IgM anti-GM2- sera. Mean + SD, Spearman’s rho rs=0.9532, p < 0.0001. **** p < 0.0001; FC: fold change