Fig. 4

Microglia internalize VGLUT2 + synapses and C3 after injury and display morphological features indicative of activation in both dLGN, and these changes are reduced by complement inhibition. A Representative microglial reconstructions with internalized VGLUT2 (green) and C3 (red), and microglial morphology (IBA1, magenta). Scale bar = 10 µm. B Microglial filament length to volume ratio, C microglial internalization of C3, and D internalization of VGLUT2 in the contralateral dLGN. (E) Microglia filament length to volume ratio, F microglial internalization of C3, and G microglial internalization of VGLUT2 in the ipsilateral dLGN. C, F t-test. B, D, E, and F one-way ANOVA with Tukey correction for multiple comparisons. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Error bars = mean ± s.e.m