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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Neuroinflammation driven by human immunodeficiency virus-1 (HIV-1) directs the expression of long noncoding RNA RP11-677M14.2 resulting in dysregulation of neurogranin in vivo and in vitro

Fig. 3

Transcriptional activation of Nrgn is associated with repression of RP11-677M14.2 lncRNA. Representative images of single molecule RNA multiplex HCR FISH with probe sets for (A) RP11-677M14.2 transcript (AF-488, green puncta) and (B) Nrgn mRNA (AF-647, red puncta) and (C) the colocalization of both transcripts in undifferentiated SH- SY5Y neuroblastoma cells. DAPI stain for cell nuclei is shown in blue. Scale bars show 10 μm, N = 3. The images are z-projections of image stacks acquired at ×60 magnification. (D) Distribution of RP11-677M14.2 transcript and Nrgn mRNA were quantified in different cellular compartments and presented as average percentage rate of total RNA puncta (N = 3). (E) Relative RP11-677M14.2 and Nrgn mRNA levels in cytoplasm or nucleus of SH-SY5Y were detected by RT-qPCR. GAPDH was used as cytoplasm control and Malat1 was used as nucleus control. (F) Schematic of SH-SY5Y differentiation with retinoic acid (RA) treatment. (G) Assessment of Nrgn mRNA and RP11-677M14.2 transcript through RT-qPCR before and after SH-SY5Y differentiation treatment. N = 3 independent experiments

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Journal of Neuroinflammation

ISSN: 1742-2094