Figure 4

Iba-1+ cells in the NFL-GCL after 15 days of unilateral laser-induced OHT. Iba-1 immunostaining. Retinal whole-mount. In naive eyes (A, B) there were ramified Iba-1+ cells (arrowhead) with varicose processes and perivascular Iba-1+ cells (arrow) with elongated morphology and thick processes. Both in contralateral eyes (C, D) and in OHT eyes (E-H) ramified Iba-1+ cells (arrowhead in C-F, H) and perivascular Iba-1+ cells (arrow in D, F) showed a retraction of the cellular processes. Scarce ameboid Iba-1+ cells were detected in contralateral eyes (D inset) and more frequently in OHT eyes (blank arrowhead in G). Only in OHT eyes were two additional morphological types of Iba-1+ cells discerned (G, H): rounded cells (asterisk in G) and rod-like cells with elongated cell bodies and two processes prominently projecting from each pole (blank arrow in H). (NFL-GCL: nerve fiber layer-ganglion cell layer; OHT: ocular hypertension; v: retinal vessel).