Table 2 PCR primers and cycling conditions for RT-PCR assay.
From: Differential regulation of Aβ42-induced neuronal C1q synthesis and microglial activation
Gene | Primer sequences | Denaturation | Annealing | Extension | cycle | Ref |
|---|---|---|---|---|---|---|
C1qB | 5'-cgactatgcccaaaacacct-3' 5'-ggaaaagcagaaagccagtg-3' | 94°C 1 min | 60°C 1 min30 sec | 72°C 2 min | 35 | [61] |
MCSF | 5'-ccgttgacagaggtgaacc-3' 5'-tccacttgtagaacaggaggc-3' | 92°C 30 sec | 58°C 1 min | 72°C 1 min30 sec | 35 | [62] |
CD40 | 5'-cgctatggggctgcttgttgacag-3' 5'-gacggtatcagtggtctcagtggc-3' | 94°C 30 sec | 58°C30 sec | 72°C 1 min | 30 | [63] |
β-actin | 5'-ggaaatcgtgcgtgacatta-3' 5'-gatagagccaccaatccaca-3' | 94°C 30 sec | 60°C30 sec | 72°C 1 min | 25 | [61] |
IL-8 | 5'-gactgttgtggcccgtgag-3' 5'-ccgtcaagctctggatgttct-3' | 94°C 1 min | 56°C 1 min | 72°C 1 min | 39 | [64] |