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Table 2 PCR primers and cycling conditions for RT-PCR assay.

From: Differential regulation of Aβ42-induced neuronal C1q synthesis and microglial activation

Gene Primer sequences Denaturation Annealing Extension cycle Ref
C1qB 5'-cgactatgcccaaaacacct-3'
5'-ggaaaagcagaaagccagtg-3'
94°C 1 min 60°C 1 min30 sec 72°C 2 min 35 [61]
MCSF 5'-ccgttgacagaggtgaacc-3'
5'-tccacttgtagaacaggaggc-3'
92°C 30 sec 58°C 1 min 72°C 1 min30 sec 35 [62]
CD40 5'-cgctatggggctgcttgttgacag-3'
5'-gacggtatcagtggtctcagtggc-3'
94°C 30 sec 58°C30 sec 72°C 1 min 30 [63]
β-actin 5'-ggaaatcgtgcgtgacatta-3'
5'-gatagagccaccaatccaca-3'
94°C 30 sec 60°C30 sec 72°C 1 min 25 [61]
IL-8 5'-gactgttgtggcccgtgag-3'
5'-ccgtcaagctctggatgttct-3'
94°C 1 min 56°C 1 min 72°C 1 min 39 [64]