Adhesion of monocytes to type I collagen stimulates an APP-dependent proinflammatory signaling response and release of Aβ1-40
© Sondag and Combs; licensee BioMed Central Ltd. 2010
Received: 15 February 2010
Accepted: 19 March 2010
Published: 19 March 2010
Amyloid precursor protein (APP) is a ubiquitously expressed cell surface protein reported to be involved in mediating cell-cell or cell-matrix interactions. Prior work has demonstrated that APP co-localizes with β1 integrin in different cell types.
In an effort to determine the function of APP on monocytic lineage cells, in particular, the human monocyte cell line, THP-1, was used to assess the role of APP during adhesion to the extracelluar matrix component type I collagen.
Pull-down assays demonstrated that THP-1 adhesion to collagen stimulated a tyrosine kinase-associated signaling response which included subsequent phosphorylation of p38 MAP kinase and increased association of APP with α2β1 integrin, specifically. In addition, cell adhesion was dependent upon APP expression since APP siRNA knockdown attenuated THP-1 adhesion to collagen compared to mock transfected controls. One consequence of the tyrosine kinase-dependent signaling response was increased secretion of interleukin-1β (IL-1β) and Aβ1-40 but not the Aβ1-42 fragment of APP. Increased secretion of IL-1β was dependent upon p38 MAP kinase activity while Aβ1-40 secretion required Src family kinase activity since the specific p38 inhibitor, SB202190, and the Src family kinase inhibitor, PP2, attenuated IL-1β and Aβ1-40 secretion, respectively.
These data demonstrate that APP is involved in classic integrin-dependent tyrosine kinase-associated adhesion and activation of peripheral monocytic cells. Moreover, divergent APP-dependent signaling is required for increased secretion of both IL-1β and Aβ1-40 as a component of the adhesion-dependent change in phenotype. This suggests that APP may have a broad role in not only mediating cell-matrix adhesion but also in the function of peripheral immune cells.
The progressive accumulation of extracellular beta amyloid-containing plaques is a cardinal feature of Alzheimer's disease (AD) pathology. The beta amyloid (Aβ) peptide is generated through sequential cleavage of the amyloid precursor protein (APP) by β- and γ-secretase. Altered proteolysis of APP due to missense mutations in the APP gene results in increased production of Aβ and increased accumulation of plaques [1–3]. APP is a ubiquitously expressed integral membrane protein with a relatively undefined function separate from generating Aβ . Several data support a role for APP in adhesion including evidence that APP co-localizes with β1 integrins in both neurons and monocytes as well as evidence that APP binds directly to type I collagen and other extracellular matrix (ECM) molecules [5–8].
Adhesion of monocytes to vascular endothelium is a highly regulated, necessary step in transendothelial migration that occurs constitutively but also during a variety of conditions including peripheral inflammation, infection, atherosclerosis, neurodegenerative disease, and injury [9–14]. Monocyte adhesion to the endothelium initiates changes in their gene expression important in differentiation and extravasation . During diapedesis, monocytes also closely interact with extracellular matrix components, in part through integrin receptor interaction, which can further activate these cells towards macrophage differentiation [15–17]. Integrin-mediated activation of monocytes is characterized by a tyrosine-kinase dependent proinflammatory signaling response and release of multiple proinflammatory molecules [7, 18–21]. Interestingly, in vitro exposure of endothelial cells to Aβ1-40 up-regulates endothelial adhesion molecule expression and monocytic adhesion to these cells, thus facilitating the transmigration process .
Plasma levels of Aβ have debatable implications with regard to increased risk for cerebral amyloid angiopathy . Aβ1-40, rather than Aβ1-42, appears to be the dominant component in the vascular depositions [24, 25]. While Aβ1-42 levels and deposition in the brain are correlated with the incidence of AD, it has been demonstrated that increased levels of plasma Aβ1-40 correlate with an increased incidence of dementia . Platelets are a known source of peripheral Aβ production and recent data shows an increased β-secretase activity in platelets from patients with AD [27, 28]. However, monocytes are another peripheral source of Aβ and interestingly it has been shown that adhesion stimulates an increased release of Aβ1-40 from these cells .
This study aims to further characterize the role of APP in adhesion-mediated activation of monocytes. In addition, we propose that β1 integrin ligation of monocytes by an endogenous ligand such as type I collagen is an important mechanism through which peripheral Aβ is released, and this may have implications in pathological accumulations of Aβ in the brain parenchyma as well as in the vasculature.
The 4G10 monoclonal anti-phosphotyrosine antibody was purchased from Upstate Biotechnology (Lake Placid, NY). Anti-β-Amyloid Precursor Protein polyclonal antibody was from Zymed Laboratories (San Francisco, CA). Anti-phospho-p38, anti-p38, anti-phospho-JNK, anti-JNK were from Cell Signaling (Beverly, MA). Anti-α2 integrin antibody, anti-α1 integrin antibody, anti-β1 integrin antibody, anti-phospho-ERK, anti-ERK2 antibody, and Protein A/G PLUS-Agarose beads were from Santa Cruz Biotechnology (Santa Cruz, CA). The 22C11 anti-APP monoclonal antibody, gamma-secretase inhibitor, and beta-secretase inhibitor were from Calbiochem (La Jolla, CA). Anti-mouse (goat), anti-rabbit (donkey), and anti-goat (donkey) horseradish peroxidase (HRP) conjugated secondary antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). APP siRNA (siGene, SMARTpool Plus) was obtained from Dharmacon (Lafayette, CO). Type I collagen was derived from rat tail following a standard protocol. All other materials not specified were purchased from Sigma (St. Louis, MO).
THP-1 cells are a monocytic cell line derived from peripheral blood of a human with acute monocytic leukemia commercially available from the American Type Culture Collection (Manassas, VA). THP-1 cells were grown in RPMI-1640 (Gibco RBL, Rockville, MD) containing 10% heat inactivated fetal bovine serum (FBS) (U.S. Biotechnologies Inc., Parkerford, PA), 5 mM Hepes, and 1.5 μg/mL pen/strep/neomycin.
THP-1 monocytes were removed from normal growth medium into serum-free RPMI and incubated at 37°C for fifteen minutes prior to stimulation. Tissue culture wells were coated with type I rat tail collagen and allowed to dry in a laminar flow hood. THP-1 cells were added to either low-adhesion tissue culture plastic alone or to wells coated with type I collagen at a density of 2 × 106 cells/mL. Cells were stimulated for either 30 min, 24 hours, or 48 hours. In inhibition studies, cells were incubated at 37°C for 30 minutes with inhibitors before stimulation.
Ice cold RIPA buffer (20 mM Tris pH 7.4, 150 mM NaCl, 1 mM Na3VO4, 10 mM NaF, 1 mM EDTA, 1 mM EGTA, 0.2 mM PMSF, 1% Triton, 0.1% SDS, 0.5% deoxycholate) was used to lyse cells. Cell lysates were sonicated and centrifuged (14,000 RPM, 4°C, 10 min) to remove insoluble material. Protein concentrations were quantitated by the method of Bradford . Proteins were resolved by 7% or 10% SDS-PAGE and transferred to PVDF membranes for Western blotting. Western blots were blocked in TBS-T (10 mM Tris, pH 7.4, 100 mM NaCl, and 0.1% Tween 20) containing 3% BSA for 15 minutes and then incubated overnight at 4°C in primary antibodies. Blots were washed three times in TBS-T, followed by incubation for one hour with HRP conjugated secondary antibodies in TBS-T containing 5% nonfat dried milk. The blots were washed three times in TBS-T followed by detection with enhanced chemiluminescence (Pierce, Rockford, IL). In some instances, blots were stripped in 0.2 N NaOH, 5 minutes, 25°C.
For co-immunoprecipitation, cells were lysed in ice cold Triton lysis buffer (20 mM Tris pH 7.4, 150 mM NaCl, 1 mM Na3VO4, 10 mM NaF, 1 mM EDTA, 1 mM EGTA, 0.2 mM PMSF, 1% Triton). Lysates were vortexed then incubated on ice for 15 minutes followed by pulse sonication. Cells were centrifuged 10 minutes at 4°C to remove insoluble material. Primary antibody (1 μg/mg protein) was added and incubated 2 hours at 4°C. Protein A/G beads (35 μl) were added and incubated 2 hours at 4°C. Beads were washed three times with lysis buffer and immunoprecipitates were resolved and Western blotted as described above.
Kinase and secretase Inhibition
4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo [3,4-d]pyrimidine (PP2) was purchased from Alexis Biochemicals (San Diego, CA). PP2 is a small-molecule inhibitor specific for Src-family kinases believed to be a mixed competitive inhibitor vis-à-vis the substrate . The cell permeable pyridinyl imidazole compound, SB202190, inhibits p38 kinase activity through competition with ATP binding (Calbiochem) . The gamma-secretase inhibitor, Z-Leu-Leu-Nle-CHO, is a cell permeable synthetic tripeptide aldehyde inhibitor of gamma secretase activity (Calbiochem) . The beta secretase inhibitor, Z-VLL-CHO; N-Benzyloxycarbonyl-val-leu-leucinal is a cell permeable, reversible inhibitor (Calbiochem) .
THP-1 cells were loaded with calcein-AM (Molecular Probes, Eugene, OR) by incubating at 1 × 106 cells/mL in serum-free RPMI 1640 medium containing 5 μg/mL calcein-AM for 30 min at 37°C. Cells were washed three times with medium and resuspended at a plating density of 1 × 106 cells/mL. 50 μl aliquots were added to a 96-well plate coated with collagen and incubated at 37°C and 5% CO2 for 45 min. Total cell number per well was then assessed by reading the plates at an excitation wavelength of 485 nm and an emission wavelength of 538 nm prior to washing the wells. Plates were then washed three times with PBS and fluorescence of adhered cells was determined at an excitation wavelength of 485 nm and an emission wavelength of 538 nm. The fluorescence value of adhered cells was then normalized according to the original cell number value.
Enzyme linked-immuno-sorbent assay (ELISA)
Media was collected from THP-1 cells following 24 hour or 48 hour stimulation. Media was centrifuged for clarification (14,000 × g, 2 min, 25°C) prior to analysis. Levels of human interleukin-1β, Aβ1-42, and Aβ1-40 in the media were determined using commercially available ELISA kits (Immuno-Biological Laboratories Inc. Minneapolis, MN), according to the manufacturer's protocol.
APP siRNA transfection
THP-1 cells were transfected with an individual APP siRNA duplex (GCCTAAAGCTGATAAGAAG) (Dharmacon, Lafayette, CO) (2 × 106 cells: 2 μg siRNA) using the appropriate Nucleofector program as described by the manufacturer (Amaxa Inc., Gaithersburg, MD).
Our data demonstrate that binding of THP-1 monocytes to type I collagen stimulates a tyrosine kinase-dependent proinflammatory signaling cascade that includes activation of p38 MAPK and subsequent increased IL-1β protein levels. In addition, monocyte adhesion to type I collagen stimulates a decrease in full-length glycosylated APP protein levels and a downstream increase in Aβ1-40 release. Furthermore, we have strengthened the existing data that suggest APP itself has a role in adhesion as an APP siRNA-induced decrease in APP protein levels resulted in a significant decrease in adhesion of monocytes to type I collagen.
We previously demonstrated that β1 integrin ligation on monocytes stimulates increased tyrosine kinase activity, recruitment of tyrosine kinases Lyn and Syk to APP, and ultimately a reactive phenotype . β1 integrins form heterodimeric receptors with different α subunits and the specific combination of αβ subunits dictates both binding specificity and overall function of the receptor . Here we identify that it is the α2 subunit that is recruited to the multi-receptor signaling complex during the APP-β1 integrin associated signaling response in monocytes . Therefore, the α2β1 integrin receptor, otherwise known as VLA-2 or CD49b, forms a complex with APP to mediate the proinflammatory activation of THP-1 monocytes upon adhesion to an endogenous ligand, type I collagen. Importantly, both APP and α2 integrin have collagen binding domains in their extracellular regions supporting our observations.
The α2β1 integrin receptor has been identified as a dual collagen/laminin receptor in several cell types . It is a major collagen receptor expressed in keratinocytes, endothelial cells, fibroblasts as well as immune cells. More interestingly, APP and its proteolytic products, have also been suggested to have a role in keratinocyte [40, 41], endothelial cell [42, 43], fibroblast [44, 45] and immune cell [7, 36, 46, 47] function. This correlation supports our observation of a coupled APP-integrin associated function in monocytes but also suggests that this biology will be relevant to a plethora of cell types. It is known that platelets express the necessary secretases to generate Aβ peptides and are suggested to be the major APP expressing cells in the blood [28, 48, 49]. Moreover, when they are stimulated, including by physiologic agonists such as thrombin and collagen, they are capable of secreting increased levels of various Aβ peptides [50, 51]. In turn, platelets can also be stimulated by Aβ1-40 to increase their aggregation . Therefore, platelets have been identified as an important peripheral source of Aβ. Although our study has focused on monocyte APP-α2β1 integrin dependent events, it is possible that this biology will be relevant to platelets as well since this receptor has a prominent role in platelet aggregation.
Integrin-dependent monocyte activation, in particular, involves a complex activation of tyrosine kinases and monocyte adhesion to this extracellular matrix protein stimulates increased cytokine and protease secretion [53–55]. Moreover, it has already been confirmed that α2β1 integrin can operate with other cell surface receptors to mediate its range of effects on phenotype [56, 57]. Similarly, our data demonstrated a dependency of α2β1 on APP expression for stimulating type I collagen-dependent increases in tyrosine kinase-dependent signaling and cellular adhesion. Therefore, monocytic functions typically attributed to α2β1 integrin may be altered in animals with decreased or mutant APP expression. For example, studies using α2 knockout animals, have demonstrated an impaired innate immune response. It is intriguing to speculate that APP is also involved in this biology and APP null animals will share a similar dysfunction of innate immune cells.
Besides the APP-α2β1-mediated adhesion of monocytes to type I collagen, we also observed a subsequent, specific processing of APP. Specifically, secreted Aβ1-42 levels remain unchanged after adhesion-mediated activation while secreted Aβ1-40 levels increased. Interestingly, Src family kinase activity regulated the collagen-stimulated increase in secreted levels of Aβ1-40 in spite of the fact that it had no ability to attenuate the decrease in full length glycosylated protein in this stimulation. This implies that tyrosine kinase activity regulates some point in the transport, turnover, secretion or release of Aβ1-40 rather than APP proteolysis. Not surprisingly, Src family kinases have a diverse role in regulating both uptake and release of membrane associated proteins. For example, cell culture observations have demonstrated a role for Src or related family members in positively regulating macropinocytosis , negatively regulating kiss and run type exocytosis , and positively regulating calveolae release from the plasma membrane through phosphorylation of calveolin-1 . Although the focus of this work has been on Aβ peptide secretion as an endpoint of tyrosine kinase mediated regulation, it is certainly of interest to the laboratory to further define what step kinase activity regulates whether it is transport, proteolysis, or turnover, in future studies.
On the other hand, p38 MAP kinase but not Src family inhibition attenuated an increase in secreted levels of collagen-stimulated release of IL-1β. This data supports the idea that a divergence in the signaling response occurs downstream of the membrane proximal tyrosine kinase activity allowing for discreet regulation of transcriptional and secretory events. Conversely, β secretase but not γ secretase inhibition significantly increased release of IL-1β during collagen stimulation. One interpretation of this result is that Aβ1-40 or some other proteolytic fragment of APP normally acts in an autocrine fashion during collagen stimulation to downregulate the magnitude of proinflammatory activation. However, since tyrosine kinase inhibition had no effect on decreasing IL-1β secretion, yet was able to decrease Aβ1-40 release, it is reasonable to assume that a proteolytic fragment generated after β secretase activity yet before γ secretase activity is involved in downregulating cytokine release. This is in agreement with prior literature demonstrating that exosome secretion of C-terminal fragments of APP can occur in vitro suggesting that they may have functions unique from Aβ or APP [62, 63]. Indeed, several studies both in vitro and in vivo have demonstrated that elevations in specific C-terminal fragments, rather than Aβ peptides, mediate cellular death and behavioral dysfunction [64–66]. Tyrosine kinase associated changes have also been linked to unique APP C-terminal fragments, as well. For example, the C99 but not the C89 fragment of APP, upon tyrosine phosphorylation, associates with the adaptor protein, ShcA, to likely propagate a unique signaling response .
Although we have not yet determined the consequences of increased monocytic Aβ1-40 secretion following adhesion-stimulated release, there are several possibilities. The secreted peptide could act upon platelets to increase their aggregation , stimulate death of endothelium  or increase endothelial proinflammatory protein expression to facilitate monocyte transmigration [22, 69]. The secreted peptide could also act in an autocrine fashion to stimulate monocytic phenotype changes favoring increased recruitment to the endothelium and subsequent adhesion . This may have some significance with respect to AD pathology as the peptide is able to cross into the brain via interaction with the receptor for advanced glycation end products (RAGE) [71, 72], but also may be important in regards to the vascular deposition of Aβ that is also evident in AD .
During periods of acute or prolonged peripheral inflammation when there is increased transendothelial migration of monocytes and increased monocytic interaction with extracellular matrix components, this adhesion may specifically perpetuate the inflammatory state by stimulating APP-dependent proinflammatory signaling in the monocytes and secretion of proinflammatory molecules such as IL-1β and Aβ1-40. Indeed, increased monocytic cell surface APP is associated with HIV-associated cognitive impairment ) and Alzheimer's disease  and cytokine and endotoxin stimulation . Prior studies have reported that monocyte to macrophage differentiation correlates with increased expression of APP  consistent with our observations that adhesion dependent activation of monocytes involves APP-integrin dependent signaling. Indeed, others have proposed a similar idea of an APP-related role in phagocyte activation by demonstrating increased expression of gamma secretase components, presenilin and nicastrin, in microglia during the gliotic response after brain injury .
These data suggest that APP has a particular role in regulating monocyte/macrophage immune cell phenotype in response to specific integrin-mediated stimulation. In addition, these findings support the hypothesis that monocyte/macrophage APP may contribute to adhesion-dependent inflammatory changes in both the brain and periphery in varying conditions including Alzheimer's disease, cardiovascular/cerebrovascular disease, and vascular amyloidosis
This work was supported by NIH 2P20RR017600 and NIH 1R01AG02633 to C.K.C.
- Citron M, Oltersdorf T, Haass C, McConlogue L, Hung AY, Seubert P, Vigo-Pelfrey C, Lieberburg I, Selkoe DJ: Mutation of the beta-amyloid precursor protein in familial Alzheimer's disease increases beta-protein production. Nature. 1992, 360: 672-4. 10.1038/360672a0.View ArticlePubMedGoogle Scholar
- Suzuki N, Cheung TT, Cai XD, Odaka A, Otvos L, Eckman C, Golde TE, Younkin SG: An increased percentage of long amyloid beta protein secreted by familial amyloid beta protein precursor (beta APP717) mutants. Science. 1994, 264: 1336-40. 10.1126/science.8191290.View ArticlePubMedGoogle Scholar
- Scheuner D, Eckman C, Jensen M, Song X, Citron M, Suzuki N, Bird TD, Hardy J, Hutton M, Kukull W, et al: Secreted amyloid beta-protein similar to that in the senile plaques of Alzheimer's disease is increased in vivo by the presenilin 1 and 2 and APP mutations linked to familial Alzheimer's disease. Nat Med. 1996, 2: 864-70. 10.1038/nm0896-864.View ArticlePubMedGoogle Scholar
- Kang J, Lemaire HG, Unterbeck A, Salbaum JM, Masters CL, Grzeschik KH, Multhaup G, Beyreuther K, Muller-Hill B: The precursor of Alzheimer's disease amyloid A4 protein resembles a cell-surface receptor. Nature. 1987, 325: 733-6. 10.1038/325733a0.View ArticlePubMedGoogle Scholar
- Yamazaki T, Koo EH, Selkoe DJ: Cell surface amyloid beta-protein precursor colocalizes with beta 1 integrins at substrate contact sites in neural cells. J Neurosci. 1997, 17: 1004-10.PubMedGoogle Scholar
- Beher D, Hesse L, Masters CL, Multhaup G: Regulation of amyloid protein precursor (APP) binding to collagen and mapping of the binding sites on APP and collagen type I. J Biol Chem. 1996, 271: 1613-20. 10.1074/jbc.271.3.1613.View ArticlePubMedGoogle Scholar
- Sondag CM, Combs CK: Amyloid precursor protein mediates proinflammatory activation of monocytic lineage cells. J Biol Chem. 2004, 279: 14456-63. 10.1074/jbc.M313747200.View ArticlePubMedGoogle Scholar
- Kibbey MC, Jucker M, Weeks BS, Neve RL, Van Nostrand WE, Kleinman HK, Kleinman HK: beta-Amyloid precursor protein binds to the neurite-promoting IKVAV site of laminin. Proc Natl Acad Sci USA. 1993, 90: 10150-3. 10.1073/pnas.90.21.10150.PubMed CentralView ArticlePubMedGoogle Scholar
- Maslin CL, Kedzierska K, Webster NL, Muller WA, Crowe SM: Transendothelial migration of monocytes: the underlying molecular mechanisms and consequences of HIV-1 infection. Curr HIV Res. 2005, 3: 303-17. 10.2174/157016205774370401.View ArticlePubMedGoogle Scholar
- da Costa Martins PA, van Gils JM, Mol A, Hordijk PL, Zwaginga JJ: Platelet binding to monocytes increases the adhesive properties of monocytes by up-regulating the expression and functionality of beta1 and beta2 integrins. J Leukoc Biol. 2006, 79: 499-507. 10.1189/jlb.0605318.View ArticlePubMedGoogle Scholar
- Sotiriou SN, Orlova VV, Al-Fakhri N, Ihanus E, Economopoulou M, Isermann B, Bdeir K, Nawroth PP, Preissner KT, Gahmberg CG, et al: Lipoprotein(a) in atherosclerotic plaques recruits inflammatory cells through interaction with Mac-1 integrin. Faseb J. 2006, 20: 559-61.PubMedGoogle Scholar
- Thomas-Ecker S, Lindecke A, Hatzmann W, Kaltschmidt C, Zanker KS, Dittmar T: Alteration in the gene expression pattern of primary monocytes after adhesion to endothelial cells. Proc Natl Acad Sci USA. 2007, 104: 5539-44. 10.1073/pnas.0700732104.PubMed CentralView ArticlePubMedGoogle Scholar
- Bradfield PF, Johnson-Leger CA, Zimmerli C, Imhof BA: LPS differentially regulates adhesion and transendothelial migration of human monocytes under static and flow conditions. Int Immunol. 2008, 20: 247-57. 10.1093/intimm/dxm136.View ArticlePubMedGoogle Scholar
- Schnoor M, Parkos CA: Disassembly of endothelial and epithelial junctions during leukocyte transmigration. Front Biosci. 2008, 13: 6638-52. 10.2741/3178.View ArticlePubMedGoogle Scholar
- Chung A, Gao Q, Kao WJ: Macrophage matrix metalloproteinase-2/-9 gene and protein expression following adhesion to ECM-derived multifunctional matrices via integrin complexation. Biomaterials. 2007, 28: 285-98. 10.1016/j.biomaterials.2006.08.038.View ArticlePubMedGoogle Scholar
- Liao F, Huynh HK, Eiroa A, Greene T, Polizzi E, Muller WA: Migration of monocytes across endothelium and passage through extracellular matrix involve separate molecular domains of PECAM-1. J Exp Med. 1995, 182: 1337-43. 10.1084/jem.182.5.1337.View ArticlePubMedGoogle Scholar
- Sudhakaran PR, Radhika A, Jacob SS: Monocyte macrophage differentiation in vitro: Fibronectin-dependent upregulation of certain macrophage-specific activities. Glycoconj J. 2007, 24: 49-55. 10.1007/s10719-006-9011-2.View ArticlePubMedGoogle Scholar
- Lin TH, Rosales C, Mondal K, Bolen JB, Haskill S, Juliano RL: Integrin-mediated tyrosine phosphorylation and cytokine message induction in monocytic cells. A possible signaling role for the Syk tyrosine kinase. J Biol Chem. 1995, 270: 16189-97. 10.1074/jbc.270.27.16189.View ArticlePubMedGoogle Scholar
- Lin TH, Yurochko A, Kornberg L, Morris J, Walker JJ, Haskill S, Juliano RL: The role of protein tyrosine phosphorylation in integrin-mediated gene induction in monocytes. J Cell Biol. 1994, 126: 1585-93. 10.1083/jcb.126.6.1585.View ArticlePubMedGoogle Scholar
- Dackiw AP, Nathens AB, Marshall JC, Rotstein OD: Integrin engagement induces monocyte procoagulant activity and tumor necrosis factor production via induction of tyrosine phosphorylation. J Surg Res. 1996, 64: 210-5. 10.1006/jsre.1996.0330.View ArticlePubMedGoogle Scholar
- Owen KA, Pixley FJ, Thomas KS, Vicente-Manzanares M, Ray BJ, Horwitz AF, Parsons JT, Beggs HE, Stanley ER, Bouton AH: Regulation of lamellipodial persistence, adhesion turnover, and motility in macrophages by focal adhesion kinase. J Cell Biol. 2007, 179: 1275-87. 10.1083/jcb.200708093.PubMed CentralView ArticlePubMedGoogle Scholar
- Gonzalez-Velasquez FJ, Moss MA: Soluble aggregates of the amyloid-beta protein activate endothelial monolayers for adhesion and subsequent transmigration of monocyte cells. J Neurochem. 2008, 104: 500-13.PubMedGoogle Scholar
- Greenberg SM, Cho HS, O'Donnell HC, Rosand J, Segal AZ, Younkin LH, Younkin SG, Rebeck GW: Plasma beta-amyloid peptide, transforming growth factor-beta 1, and risk for cerebral amyloid angiopathy. Ann N Y Acad Sci. 2000, 903: 144-9. 10.1111/j.1749-6632.2000.tb06361.x.View ArticlePubMedGoogle Scholar
- Bading JR, Yamada S, Mackic JB, Kirkman L, Miller C, Calero M, Ghiso J, Frangione B, Zlokovic BV: Brain clearance of Alzheimer's amyloid-beta40 in the squirrel monkey: a SPECT study in a primate model of cerebral amyloid angiopathy. J Drug Target. 2002, 10: 359-68. 10.1080/10611860290031831.View ArticlePubMedGoogle Scholar
- Van Dorpe J, Smeijers L, Dewachter I, Nuyens D, Spittaels K, Haute Van Den C, Mercken M, Moechars D, Laenen I, Kuiperi C, et al: Prominent cerebral amyloid angiopathy in transgenic mice overexpressing the london mutant of human APP in neurons. Am J Pathol. 2000, 157: 1283-98.PubMed CentralView ArticlePubMedGoogle Scholar
- van Oijen M, Hofman A, Soares HD, Koudstaal PJ, Breteler MM: Plasma Abeta(1-40) and Abeta(1-42) and the risk of dementia: a prospective case-cohort study. Lancet Neurol. 2006, 5: 655-60. 10.1016/S1474-4422(06)70501-4.View ArticlePubMedGoogle Scholar
- Chen M, Inestrosa NC, Ross GS, Fernandez HL: Platelets are the primary source of amyloid beta-peptide in human blood. Biochem Biophys Res Commun. 1995, 213: 96-103. 10.1006/bbrc.1995.2103.View ArticlePubMedGoogle Scholar
- Johnston JA, Liu WW, Coulson DT, Todd S, Murphy S, Brennan S, Foy CJ, Craig D, Irvine GB, Passmore AP: Platelet beta-secretase activity is increased in Alzheimer's disease. Neurobiol Aging. 2008, 29: 661-8. 10.1016/j.neurobiolaging.2006.11.003.View ArticlePubMedGoogle Scholar
- Maler JM, Spitzer P, Klafki HW, Esselmann H, Bibl M, Lewczuk P, Kornhuber J, Herrmann M, Wiltfang J: Adherence-dependent shifts in the patterns of beta-amyloid peptides secreted by human mononuclear phagocytes. Brain Behav Immun. 2008Google Scholar
- Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976, 72: 248-54. 10.1016/0003-2697(76)90527-3.View ArticlePubMedGoogle Scholar
- Hanke JH, Gardner JP, Dow RL, Changelian PS, Brissette WH, Weringer EJ, Pollok BA, Connelly PA: Discovery of a novel, potent, and Src family-selective tyrosine kinase inhibitor. Study of Lck- and FynT-dependent T cell activation. J Biol Chem. 1996, 271: 695-701. 10.1074/jbc.271.2.695.View ArticlePubMedGoogle Scholar
- Young PR, McLaughlin MM, Kumar S, Kassis S, Doyle ML, McNulty D, Gallagher TF, Fisher S, McDonnell PC, Carr SA, et al: Pyridinyl imidazole inhibitors of p38 mitogen-activated protein kinase bind in the ATP site. J Biol Chem. 1997, 272: 12116-21. 10.1074/jbc.272.18.12116.View ArticlePubMedGoogle Scholar
- Wolfe MS: Secretase targets for Alzheimer's disease: identification and therapeutic potential. J Med Chem. 2001, 44: 2039-60. 10.1021/jm0004897.View ArticlePubMedGoogle Scholar
- Abbenante G, Kovacs DM, Leung DL, Craik DJ, Tanzi RE, Fairlie DP: Inhibitors of beta-amyloid formation based on the beta-secretase cleavage site. Biochem Biophys Res Commun. 2000, 268: 133-5. 10.1006/bbrc.2000.2098.View ArticlePubMedGoogle Scholar
- Jung SS, Gauthier S, Cashman NR: Beta-amyloid precursor protein is detectable on monocytes and is increased in Alzheimer's disease. Neurobiol Aging. 1999, 20: 249-57. 10.1016/S0197-4580(99)00051-2.View ArticlePubMedGoogle Scholar
- Vehmas A, Lieu J, Pardo CA, McArthur JC, Gartner S: Amyloid precursor protein expression in circulating monocytes and brain macrophages from patients with HIV-associated cognitive impairment. J Neuroimmunol. 2004, 157: 99-110. 10.1016/j.jneuroim.2004.08.035.View ArticlePubMedGoogle Scholar
- Sondag CM, Combs CK: Amyloid precursor protein cross-linking stimulates beta amyloid production and pro-inflammatory cytokine release in monocytic lineage cells. J Neurochem. 2006, 97: 449-61. 10.1111/j.1471-4159.2006.03759.x.View ArticlePubMedGoogle Scholar
- Shenoy PS, Uniyal S, Miura K, McColl C, Oravecz T, Morris VL, Chan BM: beta1 Integrin-extracellular matrix protein interaction modulates the migratory response to chemokine stimulation. Biochem Cell Biol. 2001, 79: 399-407. 10.1139/bcb-79-4-399.View ArticlePubMedGoogle Scholar
- Elices MJ, Hemler ME: The human integrin VLA-2 is a collagen receptor on some cells and a collagen/laminin receptor on others. Proc Natl Acad Sci USA. 1989, 86: 9906-10. 10.1073/pnas.86.24.9906.PubMed CentralView ArticlePubMedGoogle Scholar
- Siemes C, Quast T, Kummer C, Wehner S, Kirfel G, Muller U, Herzog V: Keratinocytes from APP/APLP2-deficient mice are impaired in proliferation, adhesion and migration in vitro. Exp Cell Res. 2006, 312: 1939-49. 10.1016/j.yexcr.2006.02.025.View ArticlePubMedGoogle Scholar
- Schmitz A, Tikkanen R, Kirfel G, Herzog V: The biological role of the Alzheimer amyloid precursor protein in epithelial cells. Histochem Cell Biol. 2002, 117: 171-80. 10.1007/s00418-001-0351-5.View ArticlePubMedGoogle Scholar
- Ciallella JR, Figueiredo H, Smith-Swintosky V, McGillis JP: Thrombin induces surface and intracellular secretion of amyloid precursor protein from human endothelial cells. Thromb Haemost. 1999, 81: 630-7.PubMedGoogle Scholar
- Jahroudi N, Kitney J, Greenberger JS, Bowser R: Endothelial cell dysfunction in response to intracellular overexpression of amyloid precursor protein. J Neurosci Res. 1998, 54: 828-39. 10.1002/(SICI)1097-4547(19981215)54:6<828::AID-JNR11>3.0.CO;2-M.View ArticlePubMedGoogle Scholar
- Ninomiya H, Roch JM, Sundsmo MP, Otero DA, Saitoh T: Amino acid sequence RERMS represents the active domain of amyloid beta/A4 protein precursor that promotes fibroblast growth. J Cell Biol. 1993, 121: 879-86. 10.1083/jcb.121.4.879.View ArticlePubMedGoogle Scholar
- Querfurth HW, Wijsman EM, St George-Hyslop PH, Selkoe DJ: Beta APP mRNA transcription is increased in cultured fibroblasts from the familial Alzheimer's disease-1 family. Brain Res Mol Brain Res. 1995, 28: 319-37. 10.1016/0169-328X(94)00224-3.View ArticlePubMedGoogle Scholar
- Monning U, Konig G, Banati RB, Mechler H, Czech C, Gehrmann J, Schreiter-Gasser U, Masters CL, Beyreuther K: Alzheimer beta A4-amyloid protein precursor in immunocompetent cells. J Biol Chem. 1992, 267: 23950-6.PubMedGoogle Scholar
- Bullido MJ, Munoz-Fernandez MA, Recuero M, Fresno M, Valdivieso F: Alzheimer's amyloid precursor protein is expressed on the surface of hematopoietic cells upon activation. Biochim Biophys Acta. 1996, 1313: 54-62. 10.1016/0167-4889(96)00015-8.View ArticlePubMedGoogle Scholar
- Van Nostrand WE, Schmaier AH, Farrow JS, Cunningham DD: Protease nexin-II (amyloid beta-protein precursor): a platelet alpha-granule protein. Science. 1990, 248: 745-8. 10.1126/science.2110384.View ArticlePubMedGoogle Scholar
- Evin G, Zhu A, Holsinger RM, Masters CL, Li QX: Proteolytic processing of the Alzheimer's disease amyloid precursor protein in brain and platelets. J Neurosci Res. 2003, 74: 386-92. 10.1002/jnr.10745.View ArticlePubMedGoogle Scholar
- Casoli T, Di Stefano G, Giorgetti B, Grossi Y, Balietti M, Fattoretti P, Bertoni-Freddari C: Release of beta-amyloid from high-density platelets: implications for Alzheimer's disease pathology. Ann N Y Acad Sci. 2007, 1096: 170-8. 10.1196/annals.1397.082.View ArticlePubMedGoogle Scholar
- Smirnov A, Trupp A, Henkel AW, Bloch E, Reulbach U, Lewczuk P, Riggert J, Kornhuber J, Wiltfang J: Differential processing and secretion of Abeta peptides and sAPPalpha in human platelets is regulated by thrombin and prostaglandine 2. Neurobiol Aging. 2008Google Scholar
- Shen MY, Hsiao G, Fong TH, Chen HM, Chou DS, Lin CH, Sheu JR, Hsu CY: Amyloid beta peptide-activated signal pathways in human platelets. Eur J Pharmacol. 2008, 588: 259-66. 10.1016/j.ejphar.2008.04.040.View ArticlePubMedGoogle Scholar
- Pacifici R, Carano A, Santoro SA, Rifas L, Jeffrey JJ, Malone JD, McCracken R, Avioli LV: Bone matrix constituents stimulate interleukin-1 release from human blood mononuclear cells. J Clin Invest. 1991, 87: 221-8. 10.1172/JCI114975.PubMed CentralView ArticlePubMedGoogle Scholar
- Garnotel R, Rittie L, Poitevin S, Monboisse JC, Nguyen P, Potron G, Maquart FX, Randoux A, Gillery P: Human blood monocytes interact with type I collagen through alpha × beta 2 integrin (CD11c-CD18, gp150-95). J Immunol. 2000, 164: 5928-34.View ArticlePubMedGoogle Scholar
- Galt SW, Lindemann S, Medd D, Allen LL, Kraiss LW, Harris ES, Prescott SM, McIntyre TM, Weyrich AS, Zimmerman GA: Differential regulation of matrix metalloproteinase-9 by monocytes adherent to collagen and platelets. Circ Res. 2001, 89: 509-16. 10.1161/hh1801.096339.View ArticlePubMedGoogle Scholar
- Pacifici R, Basilico C, Roman J, Zutter MM, Santoro SA, McCracken R: Collagen-induced release of interleukin 1 from human blood mononuclear cells. Potentiation by fibronectin binding to the alpha 5 beta 1 integrin. J Clin Invest. 1992, 89: 61-7. 10.1172/JCI115586.PubMed CentralView ArticlePubMedGoogle Scholar
- McCall-Culbreath KD, Li Z, Zutter MM: Crosstalk between the alpha2beta1 integrin and c-met/HGF-R regulates innate immunity. Blood. 2008, 111: 3562-70. 10.1182/blood-2007-08-107664.PubMed CentralView ArticlePubMedGoogle Scholar
- Zutter MM, Edelson BT: The alpha2beta1 integrin: a novel collectin/C1q receptor. Immunobiology. 2007, 212: 343-53. 10.1016/j.imbio.2006.11.013.View ArticlePubMedGoogle Scholar
- Kasahara K, Nakayama Y, Sato I, Ikeda K, Hoshino M, Endo T, Yamaguchi N: Role of Src-family kinases in formation and trafficking of macropinosomes. J Cell Physiol. 2007, 211: 220-32. 10.1002/jcp.20931.View ArticlePubMedGoogle Scholar
- Baldwin ML, Cammarota M, Sim AT, Rostas JA: Src family tyrosine kinases differentially modulate exocytosis from rat brain nerve terminals. Neurochem Int. 2006, 49: 80-6. 10.1016/j.neuint.2006.01.002.View ArticlePubMedGoogle Scholar
- Minshall RD, Tiruppathi C, Vogel SM, Malik AB: Vesicle formation and trafficking in endothelial cells and regulation of endothelial barrier function. Histochem Cell Biol. 2002, 117: 105-12. 10.1007/s00418-001-0367-x.View ArticlePubMedGoogle Scholar
- Vingtdeux V, Hamdane M, Loyens A, Gele P, Drobeck H, Begard S, Galas MC, Delacourte A, Beauvillain JC, Buee L, et al: Alkalizing drugs induce accumulation of amyloid precursor protein by-products in luminal vesicles of multivesicular bodies. J Biol Chem. 2007, 282: 18197-205. 10.1074/jbc.M609475200.View ArticlePubMedGoogle Scholar
- Sharples RA, Vella LJ, Nisbet RM, Naylor R, Perez K, Barnham KJ, Masters CL, Hill AF: Inhibition of gamma-secretase causes increased secretion of amyloid precursor protein C-terminal fragments in association with exosomes. Faseb J. 2008, 22: 1469-78. 10.1096/fj.07-9357com.View ArticlePubMedGoogle Scholar
- Saganich MJ, Schroeder BE, Galvan V, Bredesen DE, Koo EH, Heinemann SF: Deficits in synaptic transmission and learning in amyloid precursor protein (APP) transgenic mice require C-terminal cleavage of APP. J Neurosci. 2006, 26: 13428-36. 10.1523/JNEUROSCI.4180-06.2006.View ArticlePubMedGoogle Scholar
- Rockenstein E, Mante M, Alford M, Adame A, Crews L, Hashimoto M, Esposito L, Mucke L, Masliah E: High beta-secretase activity elicits neurodegeneration in transgenic mice despite reductions in amyloid-beta levels: implications for the treatment of Alzheimer disease. J Biol Chem. 2005, 280: 32957-67. 10.1074/jbc.M507016200.View ArticlePubMedGoogle Scholar
- Cao X, Sudhof TC: Dissection of amyloid-beta precursor protein-dependent transcriptional transactivation. J Biol Chem. 2004, 279: 24601-11. 10.1074/jbc.M402248200.View ArticlePubMedGoogle Scholar
- Repetto E, Russo C, Venezia V, Nizzari M, Nitsch RM, Schettini G: BACE1 overexpression regulates amyloid precursor protein cleavage and interaction with the ShcA adapter. Ann N Y Acad Sci. 2004, 1030: 330-8. 10.1196/annals.1329.041.View ArticlePubMedGoogle Scholar
- Suo Z, Fang C, Crawford F, Mullan M: Superoxide free radical and intracellular calcium mediate A beta(1-42) induced endothelial toxicity. Brain Res. 1997, 762: 144-52. 10.1016/S0006-8993(97)00383-1.View ArticlePubMedGoogle Scholar
- Giri R, Shen Y, Stins M, Du Yan S, Schmidt AM, Stern D, Kim KS, Zlokovic B, Kalra VK: beta-amyloid-induced migration of monocytes across human brain endothelial cells involves RAGE and PECAM-1. Am J Physiol Cell Physiol. 2000, 279: C1772-81.PubMedGoogle Scholar
- Fiala M, Zhang L, Gan X, Sherry B, Taub D, Graves MC, Hama S, Way D, Weinand M, Witte M, et al: Amyloid-beta induces chemokine secretion and monocyte migration across a human blood--brain barrier model. Mol Med. 1998, 4: 480-9.PubMed CentralPubMedGoogle Scholar
- Deane R, Wu Z, Zlokovic BV: RAGE (yin) versus LRP (yang) balance regulates alzheimer amyloid beta-peptide clearance through transport across the blood-brain barrier. Stroke. 2004, 35: 2628-31. 10.1161/01.STR.0000143452.85382.d1.View ArticlePubMedGoogle Scholar
- Deane R, Sagare A, Zlokovic BV: The role of the cell surface LRP and soluble LRP in blood-brain barrier Abeta clearance in Alzheimer's disease. Curr Pharm Des. 2008, 14: 1601-5. 10.2174/138161208784705487.PubMed CentralView ArticlePubMedGoogle Scholar
- Attems J, Quass M, Jellinger KA, Lintner F: Topographical distribution of cerebral amyloid angiopathy and its effect on cognitive decline are influenced by Alzheimer disease pathology. J Neurol Sci. 2007, 257: 49-55. 10.1016/j.jns.2007.01.013.View ArticlePubMedGoogle Scholar
- Bauer J, Konig G, Strauss S, Jonas U, Ganter U, Weidemann A, Monning U, Masters CL, Volk B, Berger M, et al: In-vitro matured human macrophages express Alzheimer's beta A4-amyloid precursor protein indicating synthesis in microglial cells. FEBS Lett. 1991, 282: 335-40. 10.1016/0014-5793(91)80508-Z.View ArticlePubMedGoogle Scholar
- Nadler Y, Alexandrovich A, Grigoriadis N, Hartmann T, Rao KS, Shohami E, Stein R: Increased expression of the gamma-secretase components presenilin-1 and nicastrin in activated astrocytes and microglia following traumatic brain injury. Glia. 2008, 56: 552-67. 10.1002/glia.20638.View ArticlePubMedGoogle Scholar
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