In vivo CHI3L1 (YKL-40) expression in astrocytes in acute and chronic neurological diseases

Table 2 CHI3L1 ISH in acute, subacute and chronic infarcts.

Condition	Case No.	Age and sex	CHI3L1 ISH signal intensity
Acute infarct	1	58 female	+++
	2	61 male	++++
	3	79 female	++++
	4	85 male	++
Subacute infarct	1	56 female	+++
	2	81 female	0
	3	70 female	+
	4	89 female	+/-
	5	89 male	+++
Chronic infarct	1	88 female	+
	2	74 male	+
	3	45 male	++
	4	77 female	0
	5	88 female	0

Six-μm-thick paraffin-embedded sections were hybridized with ³⁵S-labeled RNA probe for CHI3L1 as described in Methods. ISH signal intensity was scored in 4 cases of acute infarct, 5 cases of subacute infarcts and 5 cases of chronic infarct (intense signal (++++), strong signal (+++), moderate signal (++), weak signal (+), occasional positive cells (+/-) and no signal (0)).

ISSN: 1742-2094